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Seminar INFLPR, Miercuri, 4 Decembrie 2019, ora 11:00, Secţia Laseri, Dr. I. ANTOHE: Dezvoltarea unui dispozitiv inovativ cu fibră optică bazat pe rezonanță plasmonică de suprafață (FO-SPR) pentru monitorizarea în timp real a interacţiilor bio-/moleculare

Home Information Research Events Seminars Seminar INFLPR, Miercuri, 4 Decembrie 2019, ora 11:00, Secţia Laseri, Dr. I. ANTOHE: Dezvoltarea unui dispozitiv inovativ cu fibră optică bazat pe rezonanță plasmonică de suprafață (FO-SPR) pentru monitorizarea în timp real a interacţiilor bio-/moleculare

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Seminar INFLPR

Miercuri, 4 Decembrie 2019, ora 11:00

Secţia Laseri, sala de seminar

Contractul nr. 16N /2019

Proiectul: PN 15 15 01 01, „Cercetări emergente de laseri, plasmă, radiaţii şi aplicaţiile lor în domeniile de specializare inteligentă şi interes public”

Faza nr. 17: „Dezvoltarea unui dispozitiv inovativ cu fibră optică bazat pe rezonanță plasmonică de suprafață (FO-SPR) pentru monitorizarea în timp real a interacţiilor bio-/moleculare”

Termen: 14.11.2019

Responsabil fază: Dr. Gabriel SOCOL/ Dr. Iulia ANTOHE

Prezintă: Dr. Iulia ANTOHE

Abstract: Plasmonii de suprafață (SP) sunt unde electromagnetice care se formează de-a lungul unei interfețe plasmonice, dintre un film subțire metalic (eg. aur) și un mediu dielectric (soluția de analizat). Dispozitivele bazate pe rezonanța plasmonilor de suprafață (SPR) sunt instrumente optice bine-cunoscute, cu o mare aplicabilitate în biodetecție deoarece permit monitorizarea în timp real a interacțiunilor biochimice. Scopul principal al proiectului a fost de a dezvolta în INFLPR o platformă inovativă de detecţie cu fibră optică bazată pe rezonanţa plasmonică de suprafaţă (FO-SPR). Acești senzori FO-SPR reprezintă o alternativă la sistemele clasice SPR bazate pe prismă optică, aducând avantaje cum ar fi simplitatea, portabilitatea și reducerea costurilor. Fibra optică (FO) acoperită cu un film subțire de aur este folosită pentru a transporta lumina către interfața plasmonică. Orice eveniment care are loc la această interfață (eg. interacţia dintre un receptor şi molecula ţintă specifică) va declanșa o modificare a semnalului SPR, ce poate fi procesată mai departe într-un grafic. Platforma de detecție FO-SPR dezvoltată are o sensibilitate de 1877 nm/RIU şi a fost folosită cu succes la monitorizarea în timp real a interacţiei dintre coenzima-biotină şi proteina specifică-streptavidină. În viitorul apropiat, potențialul acestui dispozitiv va fi evaluat prin implementarea unor abordări noi bazate pe schimbarea stării suprafeței plasmonice a senzorului FO-SPR, pentru a ridica această tehnologie la noi nivele de performanță, portabilitate și aplicații.

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