Intravital multiphoton imaging dissecting spatiotemporal heterogeneity of in vivo immune systems

Seminar General

Marti, 24 Septembrie 2024, ora 16:00

CETAL, Sala de seminar

Titlu: Intravital multiphoton imaging dissecting spatiotemporal heterogeneity of in vivo immune systems

Prezintă: Prof. Masaru Ishii, M.D., Ph.D., Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University
Director, Nikon Imaging Center Osaka/Japan

Abstract: During the last decade, intravital multiphoton microscopy has launched a new trend in the field of biology. By elaborating this advanced imaging technique, I have established a new system for visualizing in situ behavior of a diversity of living cells within intact tissues and organs.
Among them, I first succeeded in visualizing inside the bone tissues, where various kinds of immune and hematopoietic cells are produced and functioning although poorly analyzed by conventional methodology such as histological analyses. In addition, intravital imaging has enabled us to grasp in situ behaviors of local immune response in vivo, especially focusing on lung and liver, leading to the identification of novel pathogenic cell types only visible in native systems. In this seminar, I will show our imaging techniques with multiphoton microscopy with the latest advances in imaging biology, especially focusing on spatiotemporal heterogeneity/variability of immune systems in vivo.

References:
1) Miyamoto et al., Periportal macrophages protect against commensal-driven liver inflammation. Nature 629: 901-909, 2024.
2) Matsui et al., Nonlinear optics with near-infrared excitation enable real-time quantitative diagnosis of human cervical cancers Cancer Res. 80: 3745-3754, 2020.
3) Hasegawa et al., Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1. Nature Immunol. 20: 1631-1643, 2019.
4) Maeda et al., Real-time intravital imaging of pH variation associated with osteoclast activity. Nature Chem. Biol. 12: 579-85, 2016.
5) Ishii et al., Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis. Nature 458: 524-528, 2009.